Product Overview

MicroCal PEAQ ITC Automated combines the high sensitivity of the MicroCal PEAQ ITC with walkaway automation to meet the productivity needs of busy research and drug discovery laboratories. Temperature controlled 4 x 96 well plates and user guided software together enable exceptional sensitivity and high quality data with robust operation. ITC is an essential tool in drug discovery and enables the study of biomolecular interactions as well as changes in conformation such as protein unfolding. It can also be used to measure enzyme kinetics.

MicroCal PEAQ ITC Automated measures heat released or absorbed during biochemical binding events to calculate binding affinity (K D ), stoichiometry (n), enthalpy (ΔH) and entropy (ΔS) in a single experiment. The system uses Isothermal Titration calorimetry (ITC), a technique that requires NO labelling and minimal assay development.

The PEAQ ITC micro calorimeter comprises two cells — a sample cell in which the titration experiment is performed, and a reference cell which contains only water. A series of small aliquots of ligand are injected into the sample or protein solution. If binding occurs between the ligand and protein, heat changes of a few millionths of a degree Celsius are detected and measured until the binding reaction has reached equilibrium. The quantity of heat measured is directly proportional to the amount of binding which helps the user understand the nature of the interaction.