Avestin Emulsiflex B15

The Avestin Emulsiflex B15 cell disruptor is an air powered, French Press style, high pressure Homogeniser that rapidly disrupts cells while avoiding over processing and protein damage.

Avestin Emulsiflex B15

The Avestin Emulsiflex B15 cell disruptor is an air powered, French Press style, high pressure Homogeniser that rapidly disrupts cells while avoiding over processing and protein damage.

Manufacturer Avestin
Product SeriesEmulsiflex
Measurement principleHigh Pressure Homogenisation
ApplicationCell Lysis - Rupture
Sample typeCell rupture, emulsions
Homogenising pressureup to 45,000 psi (310 MPa)
Throughput15ml
Minimum sample volume3ml
Valve typedynamic - pneumatically controlled
Temperature controlno
Extruder optionno

Product Overview

High pressure homogenisation is an efficient and easy to use technique for cell disruption. The principle is to pump the sample through a valve with a small aperture. The sample goes from very high pressure on the pump side of the valve to atmospheric pressure on the outlet side of the valve. This extreme change of pressure produces very high shear forces on the sample as it exits the valve. The effect on cells is to disrupt their membranes.

The Emulsiflex B15 uses a dynamic homogenising valve. The benefit of a dynamic valve is that the samples are processed at a constant homogenising pressure. Most cells are ruptured in a single pass thereby avoiding over processing and protein damage.

The homogenising pressure is adjustable between 500 and 45,000psi. It has a capacity of 15mL and it can process a sample as small as 3mL.The EmulsiFlex-B15 has no “O”-rings or gaskets in the product path. It is suitable for clean room and GMP manufacturing. The wetted parts are autoclavable and it is steam-in- place (SIP) sterilisable.

The EmulsiFlex-B15 has no “O”-rings or gaskets in the product path. It is suitable for clean room and GMP manufacturing. The wetted parts are autoclavable and it is steam-in- place (SIP) sterilisable.

This cell disruptor has an air driven pump which means that no electrical power is needed. It requires a supply of compressed or bottled air. For most emulsions, liposomes and E. coli rupture, an air pressure of 100psi is sufficient.

  • Benefits
  • Applications

Benefits

Efficient and uniform processing
Most cells are ruptured in a single pass avoiding over processing and protein damage.

Compact unit
The EmulsiFlex-B15 has a capacity of 15mL and it can process a sample as small as 3mL.

No “O” rings or gaskets
There are no “O” rings or gaskets in the entire product path, which means little or no cross contamination.

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Applications

Emulsions/ Cell rupture: Pharmaceutical emulsions and cells such as yeast can be processed to a reduced size and narrow distribution.

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