Malvern Panalytical WAVEsystem

The Malvern Panalytical WAVEsystem enables high-sensitivity, label-free analysis of binding kinetics and affinity analysis for drug discovery.

Malvern Panalytical WAVEsystem

The Malvern Panalytical WAVEsystem enables high-sensitivity, label-free analysis of binding kinetics and affinity analysis for drug discovery.

Manufacturer Malvern Panalytical
Product Series WAVEsystem
Measurement principle Grating-Coupled Interferometry (GCI)
Application Biomolecular Interactions, Protein Interactions & Binding Affinity
Sample type Suitable for Fragments, Small Molecules, Peptides, Proteins, Viruses, Cell Culture Supernatants, Serums, Cell Iysates
Temp range15 °C – 40 °C (WAVE); 4 °C – 45 °C (WAVEdelta)
Flow Channels / Buffer2- channel/1 buffer (WAVE); 4-channel/ 4 buffers (WAVEdelta)
Sample Capacity2x microtiter plates (96 or 384 well, standard or deep well) or vial racks (48 positions of 1.5ml)
Information ProvidedKinetic and affnity data (ka, kd, KD)

Product Overview

The WAVEsystem provides rapid, label-free and high-sensitivity analysis to study real-time biological interactions providing deeper insight into the affinity and kinetics of the interaction as well as binding specificity.

Engineered around the patented Grating-Coupled Interferometry (GCI) technology, a range of analytes can be investigated in just one system to manage a broader range of samples than traditional SPR equipment.  This extremely versatile technology can screen crude and complex samples involving fragment, small molecules, peptides, membrane proteins, biologics and other molecules even in crude reaction mixtures and biofluids like undiluted serum and plasma.

Large molecules with high affinity and slow dissociation can be measured simply (even native proteins in complex matrices) without purification. This is made possible by WAVEchip, a microfluidics pathway that is fully contained in single, disposable 2- or 4 channel cartridge complemented by integral no-clog technology. 

With ultra-fast transition times (up to 150ms), reliable determination of off-rates of 10s-1 can be calculated for the kinetic study of small and weak binding fragments. This is aided by monitoring through the waveguide so that an increased number of binding interactions can contribute to the overall signal.

The waveRAPID (Repeated Analyte Pulses of increasing duration) method can also be used to increase throughput compared to multi-cycle kinetics, particularly in screening applications, by generating a pulsating concentration profile.

Easy-to-use software, WAVEcontrol, makes data interpretation and evaluation straight forward. The kinetic series can be evaluated in just one click, as the software automatically adjusts the data and initiates the evaluation process. Smart wizards extend the automated capabilities of the system and include: Ligand screening – a faster, flexible way to screen and characterise antibodies and Calibration-free concentration analysis (CFCA) – a reliable, quick and easy calibration-free approach to quantify active protein concentration.

  • Demonstration video
  • Benefits
  • Applications

Demonstration video


  • All sizes welcome. Screen, rank and characterise small analytes and fragments, including weak binders with fast dissociation rates (koff as high as 10/sec).
  • Kinetic profiling closer to real life. Assess drug performance in undiluted human blood serum and plasma samples.
  • Broadest kinetic range. Quantify binding affinities (Kd) from low pM to high uM with confident kinetic analysis.
  • More than buffer. Explore more solubilisation and purification conditions for membrane proteins, including viscous detergents, less common solvents, and different additives.
  • No clogging, regardless of the size. Analyse and characterise larger molecules (VLPs, liposomes) and aggregates (fibrils) without clogging-risk.
  • Tight binders welcome. Measure kinetics of high-affinity binders at low pM affinity (Kd) values.


  • Screening: off-rate, primary, fragment-based, small molecule campaigns.
  • Ligand interaction with membrane protein studies.
  • Pharmacology: kinetics in biofluids (blood serum, plasma) for drug potency assessment.
  • Biologics: characterisation and quantification of antibodies at low limits of detection in biofluids or media.
  • For more application notes click here

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