Malvern MicroCal PEAQ-DSC Automated

MicroCal PEAQ-DSC Automated is a highly sensitive, fully automated Differential Scanning Calorimeter (DSC) that provides insights into biomolecular stability without labelling or use of artificial probes.

Malvern MicroCal PEAQ-DSC Automated

MicroCal PEAQ-DSC Automated is a highly sensitive, fully automated Differential Scanning Calorimeter (DSC) that provides insights into biomolecular stability without labelling or use of artificial probes.

Manufacturer Malvern Panalytical
Product SeriesMalvern MicroCal DSC
Measurement principleDifferential Scanning Calorimetry (DSC)
ApplicationBiomolecular Interactions
Sample typeProteins, biomolecules
Sample throughputup to 50/ 24h (unattended)
Working cell volume130µL
Typical sample concentration0.01 to 10 mg/mL
Maximum scan rate240 ºC/h
Temperature range2ºC to 130ºC
Typical time per scan35 to 55 min
Maximum scans per day~50 in 24h (unattended)
Response time5s
Sample capacity288 (6 x 96-well plates)

Product Overview

MicroCal PEAQ-DSC Automated is a highly sensitive, fully automated, high-throughput Differential Scanning Calorimeter for characterising protein and biomolecule stability. It has an active cell volume of just 130 µL, allowing thermodynamic measurements of even precious samples. For high throughput work, a fully integrated auto-sampler enables running of up to 50 samples per day. All filling, injection, and cell cleaning functions are fully automated for walk-away operation.

Differential Scanning Calorimetry generates a thermodynamic profile to provide insight into the factors that affect conformation and stability. During a measurement, the reference and sample cells are heated at a constant scan rate which causes the molecule to undergo a conformational change such as unfolding. This results in the absorption of heat with the midpoint (Tm) indicating stability and the area under the curve (ΔH) giving the enthalpy of the process.

Typical applications include determining protein stability and the thermodynamics of unfolding, antibody domain structure determination and the characterisation of membranes, lipids and detergent micellar systems.

  • Benefits
  • Applications

Benefits

Label Free
Molecules can be studied in their native state without labelling. It requires minimal assay development and is ideally suited for solutions that interfere with optical measurements including turbid, coloured or particulate samples.

Excellent chemical resistance
Nonreactive Tantalum cells ensure excellent chemical resistance and inertness when working with proteins and other biomolecules.

High throughput and sensitivity with low sample consumption
Active cell volume of 130 µL, with concentration requirements as low as 0.01 mg/mL, allows thermodynamic measurement of scarce or precious samples. Matched capillary cells provide fast scan rates and fast temperature equilibration. Auto-sampler enables walk away operation for up to 50 samples to be screened per day.

Intuitive software
Designed to streamline workflow by providing simplified experimental setup. A range of thermodynamic fitting models support a variety of applications and further aid data analysis and interpretation.

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Applications

  • Characterising and selecting the most stable protein or biotherapeutic candidate in drug discovery
  • Eliminate protein or biotherapeutic candidates likely to have long-term stability issues
  • Optimising expression, purification and manufacturing conditions in drug discovery
  • Rapidly and easily determining the conditions for liquid formulation in drug discovery
  • Using DSC to study antibody Fab region stability for improved biotherapeutic development
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