02 9541 3500LUNA-STEM Fluorescence Cell Counter for Stem Cells & SVF
The LUNA-STEM takes adipose-derived stem cell and SVF samples to count live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.
LUNA-STEM Fluorescence Cell Counter for Stem Cells & SVF
The LUNA-STEM takes adipose-derived stem cell and SVF samples to count live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.
| Manufacturer | Logos Biosystems |
|---|---|
| Product Series | LUNA |
| Measurement principle | Microscopy - Cell image analysis |
| Application | Fluorescence Imaging, Live cell image analysis |
| Sample volume | 10 μl |
| Cell counting time | 30 sec |
| Cell size range | 1 to 90 μm |
| Ex/Em wavelength | 470±20 nm/ 530±25 nm, 600 nm(LP) |
Product Overview
The LUNA-STEM is an automated, compact cell counting and viability analysis system that combines dual fluorescence optics and image analysis software. Its interactive touchscreen makes accurate cell counting a simple experience.
Using Acridine Orange (AO) and Propidium Iodide (PI) to stain live and dead cells, respectively, the LUNA-STEM™ provides highly sensitive and accurate results for most cell types. Adipose-derived stem cell and SVF samples can be counted as live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.
Designed for cost-efficient and accurate cell counting, the LUNA Reusable Slide has the affordability of manual cell counting without the associated subjectivity and time. Photon Slides are disposable precision slides made with optical quality materials for optimal fluorescence signal detection. The slides offer the ultimate counting experience with no mess or clean up, while maintaining the highest standard of cell counting accuracy.
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- DEMONSTRATION VIDEO
- BENEFITS
- APPLICATIONS
DEMONSTRATION VIDEO
BENEFITS
TWO COUNTERS IN ONE
LUNA-STEM can count in both brightfield and fluorescence mode. Built-in software automatically generates cell viability data upon cell counting. For validation purposes, live and dead cells are tagged with green and red circles, respectively
COMPATIBLE WITH STEM CELLS & CELLS FROM STROMAL VASCULAR FRACTION (SVF)
The various cells within the SVF can be recognised and counted as non-nucleated cells or live and dead nucleated cells simultaneously, which may not be possible with other cell counters.
FAST COUNTING & IMAGING
Images generated from three channels in 30-60 seconds.
USER-FRIENDLY INTERFACE
Customise and save protocols, verify results immediately onboard, use the dilution calculator, and gate cells on histograms with a few taps on the LUNA-STEM touchscreen.
SIMPLE RECORDKEEPING
Print data with an external printer or export high-resolution images, data and full data reports via USB.
THE LOWEST COST, THE GREATEST CONVENIENCE
Perfect for labs on a budget, the LUNA works with reusable slides, delivering the affordability of manual cell counting without the associated time, subjectivity and user-to-user variability. The disposable LUNA Cell Counting Slides offer accurate cell counting with no mess or cleanup. PhotonSlides are made with optical quality materials with ultra-low autofluorescence to allow for optimal fluorescence detection.
APPLICATIONS
- Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue. 2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.
- Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation. 2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.
- Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials. 2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.
- Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia. 2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.
- The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging. 2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.
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