LUNA-FL Dual Fluorescence cell counter

Logos Biosystems LUNA-FL with dual fluorescence and brightfield optics accurately determines cell count, cell viability and GFP transfection efficiency without being limited by cell type or size.

LUNA-FL Dual Fluorescence cell counter

Logos Biosystems LUNA-FL with dual fluorescence and brightfield optics accurately determines cell count, cell viability and GFP transfection efficiency without being limited by cell type or size.

Manufacturer Logos Biosystems
Product Series LUNA
Measurement principle Microscopy - Cell image analysis
Application Fluorescence Imaging, Live cell image analysis
Cell counting time30 sec
Cell size range1 to 90 μm
Ex/Em wavelength470±20 nm/ 530±25 nm, 600 nm(LP)

Product Overview

LUNA-FL enables automated cell counting and cell viability analysis. This stand-alone, compact instrument combines brightfield and dual fluorescence optics to enable four counting modes: Brightfield cell counting; fluorescence cell counting; yeast cell counting and GFP transfection assays.

Integrated dual fluorescence microscope optics, allow primary cells such as PBMCs, splenocytes, neutrophils and stem cells to be distinguished from undesirable debris, usually confused for cells with conventional cell counters.

Whether counting live and dead cells with trypan blue staining or performing GFP transfection analysis using fluorescently stained cells, LUNA-FL provides the most sensitive cell analysis results.  A histogram displays live and dead cell populations allowing counted cells to be gated based on cell size information.

Designed for cost-efficient and accurate cell counting the LUNA automated cell counter is compatible with both reusable and disposable cell counting slides to maintain the highest standard of cell counting accuracy.

The LUNA family of automated counters has been awarded a Platinum seal of quality from SelectScience in recognition of outstanding feedback received from scientists globally – one of just 6 winners since the awards were launched in 2017! 

  • DEMONSTRATION VIDEO
  • BENEFITS
  • APPLICATIONS

DEMONSTRATION VIDEO

BENEFITS

TWO COUNTERS IN ONE
LUNA-FL can count in both brightfield and fluorescence mode. Dye exclusion based cell counting with brightfield optics.

COMPATIBLE WITH MOST CELLS
Advanced imaging software and GFP and RFP fluorescence optics detect fluorescence stained cells with high sensitivity. Optimised for primary cells, stem cells, PBMCs, Splenocytes, etc.

GFP TRANSFECTION ANALYSIS
High-resolution images are captured and analysed to detect GFP-positive and -negative cells.

MAXIMUM 30 SECONDS
Fluorescence: 30 s = 3 images captured & analyzed; Brightfield: 10 s = 1 image captured & analysed.

ADVANCED CLUSTER ANALYSIS
An advanced declustering algorithm detects individual cells in clusters.

USER-FRIENDLY INTERFACE
Customise and save protocols, verify results immediately onboard, use the dilution calculator, and gate cells on histograms with a few taps on the LUNA-FL touchscreen.

SIMPLE RECORDKEEPING
Print data with an external printer or export high-resolution images, data and full data reports via USB.

THE LOWEST COST, THE GREATEST CONVENIENCE
Perfect for labs on a budget, the LUNA works with reusable slides, delivering the affordability of manual cell counting without the associated time, subjectivity and user-to-user variability. The disposable LUNA Cell Counting Slides offer accurate cell counting with no mess or cleanup. PhotonSlides are made with optical quality materials with ultra-low autofluorescence to allow for optimal fluorescence detection.

APPLICATIONS

Count cell suspensions with cell sizes ranging from 1 to 90 μm. Applications include Primary cells (Splenocyte, Thymocyte, PBMC, Monocyte, Lymphocyte, Neuronal cell, Keratinocyte, Epithelial cell, Dendritic cell, etc), Cancer cells, Stem cells, Adipocytes, Sperm cells, Somatic cells in milk, Algae, Yeasts and more.

Cell Lines validated
On the LUNA-FL Automated cell counter
Cell Type Animal Organ
PBMC Mouse Blood
Splenocyte Mouse Spleen
Thymocyte Mouse Thymus
A431 Human Skin
CHO-M1WT2 Chinese Hamster Ovary
CHSE Fish Embryo
COLO-205 Human Colon
Cos-7 Human Kidney
HEK-293 Human Kidney
HeLa Human Cervix
HepG2 Human Liver
HL-60 Human Blood
J774A.1 Mouse Blood
Jurkat Human Blood
MCF7 Human Breast
MRC-5 Human Lung
NIH/3T3 Mouse Embryo

Product Enquiry

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