Measuring Change in Secondary Structure and Oligomeric State for a mAb in Stress and In-Process Testing with MMS and SEC
21 Nov, 2022 | Newsletters
Microfluidic Modulation Spectroscopy (MMS) is a new type of Mid-Infrared (MIR) spectroscopy with better sensitivity than traditional MIR and the ability to automate the measurement of samples over a large concentration range. In this talk, we examine the use of MMS to determine the secondary structure of stressed and in-process mAb samples and link these changes to aggregation of the target mAb as seen by size-exclusion chromatography (SEC). Speaker: Daniel Myatt, PhD – Senior Scientist, Centre for Process Innovation (UK) WATCH NOW A Structural Characterisation of a Monoclonal Antibody Therapeutic (Trastuzumab) as Formulated and Under Multiple Conjugation Paradigms In this study, the commercial mAb Trastuzumab was characterized by MMS directly in its formulation buffer to understand the stand-alone higher-order structure for this important mAb therapeutic. In addition, Trastuzumab was characterized at a variety of different drug-antibody ratios utilizing both non-specific labeling and click-chemistries for the drug conjugation to understand their effects on the mAb structure. Finally, the MMS results were compared to other biophysical techniques such as DSC. DOWNLOAD POSTER |
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NEW Behind the App Note Video Detection of Pressure-Induced Protein Aggregation Using Microfluidic Modulation Spectroscopy (MMS) The ability to detect protein aggregation is important at all stages of drug development. Early detection of protein aggregation is most desirable to inform development decisions, as it is a recognized signal of instability and can lead to the loss of protein function. Pressure, a stressor used for generating aggregates by impacting noncovalent interactions without the need to change temperature or solvents, was employed to create aggregated human gamma-globulin for this spiked study. WATCH NOW – CLICK HERE |