Determine binding affinity and stoichiometry of protein interactions in complex backgrounds

30 Oct, 2020 | Newsletters
Determine binding affinity and stoichiometry of protein interactions in complex backgrounds

What’s Microfluidic Diffusional Sizing & what makes it so unique? 

The Fluidity One-W with Microfluidic Diffusional Sizing (MDS) offers new way to study protein–protein interactions.
•    Study proteins in complex backgrounds — like cell lysates or blood plasma. Means minimal sample preparation is required.
•    No surfaces required — Meaning there is no surface artefacts so you can study proteins in near native state.
•    Stoichiometric analysis — When used in combination with protein modelling software hydrodynamic radius (Rh) can be used to calculate the stochiometry of complexes.

If you’re interested, we’d love to offer you a demonstration, so you can see it’s full capabilities in action. Contact us using the link below.

Accurate solution phase affinity profiling of a SARS-CoV-2 antibody in serum  
Here we show how MDS on the Fluidity One-W Serum we can accurately detect and characterise the binding affinity of antibodies to virus proteins directly in human serum, to support the development of reliable antibody tests and vaccines in the fight against the COVID-19 pandemic.

All antibodies are NOT created equal: Comprehensive profiling of SARS-CoV-2 antibodies
This talk outlines our efforts in assessing the immune response in COVID-19 patients by use of MDS. With this new approach, we were able to comprehensively profile SARS-CoV-2 antibodies directly in minimally diluted serum of these patients.

Microfluidic Diffusional Sizing (MDS) – How it works  

Microfluidic Diffusional Sizing (MDS) offers a new way to study protein interactions in crude biological backgrounds (e.g. cell lysates or blood plasma) and interactions of proteins with other secondary binding partners, like lipids and DNA. 


ATA Scientific has developed a COVID-19 Customer Response Plan to help those that require analytical testing services adapted to the remote working environment.
 Our technical support and service team is ready to support you remotely with web based demonstrations, online operator training, service support, contract sample testing and instrument hire.
Click here to read more