Demonstration video

Benefits

Quick install & analysis
NanoSight Pro can be used by every user in every lab. Smart installation allows you to get up and running quickly. With minimum setup, easy operation and intuitive NS Xplorer software you can measure your samples in minutes.

Intelligent & automated
Powered by machine learning and intelligent features, NanoSight Pro automates processing and eliminates human error, delivering superior particle identification and precise particle tracking for the highest quality and reproducible data.

Highest resolution
The NanoSight Pro measures nanoscale particles in the range of 10 nm – 1000 nm including low scattering and biological particles. Particle–by–particle analysis assures high resolution size and concentration data with visual confirmation.

Power of fluorescence
In addition to light scatter, fluorescence signals can be detected from fluorescently tagged biologics or synthetic particles providing even greater insight into your sample. Interchangeable laser modules with the choice of four excitation wavelengths can be used along with the matching long pass filters for the optimum setup supported by analysis in flow for better sampling and tracking of even photobleaching samples. The greater fluorescence sensitivity enables subpopulation identification including surface markers, internal cargo detection or depreciation of contaminants sensitivity.

Applications

Extracellular Vesicles (EV)

Whilst the research into the generation and functions of extracellular vesicles is still evolving, it is important to monitor and control their isolation and purification. NanoSight NTA provides quick and easy characterisation of both the size and concentration of vesicles in aqueous buffers, giving confidence in the quality of the sample used in any downstream experiments, whilst the use of fluorescence can assist in elucidating the origin subpopulations of EVs.

Virus and Vaccines

Manufacture of vaccines requires controlled production processes to ensure that the materials can be appropriately dosed and recognized by the immune system. NTA assists in the optimization of manufacturing processes by ensuring the size distribution of the vaccine is maintained. As NTA is also able to count and size viruses, it can be used as a faster alternative to titer assays.

Drug Delivery & Gene Therapy

With drug delivery and gene therapy products, 70-150 nm is the typical size of particles required to ensure effective disease targeting in the clinical. NTA is ideally suited to study the size of these particles from early stage research through to candidate screening, formulation development and clinical batch monitoring whilst the concentration measurement allows for dose determination of the final product as well as for use in in vitro and in vivo assays.

Biotherapeutics

Temperature, pH change, agitation, shear and time all impact the stability of biotherapeutic proteins, causing denaturation and aggregation which can cause loss of efficacy and the potential for an unwanted immune response. NTA provides high resolution size distributions of sub-visible aggregates in a formulation for safety and quality assurance.

Nanomaterials/colloids/toxicology

As nanomaterials are incorporated in to everyday items, they are coming to the attention of regulatory bodies. There is a requirement to fully characterize these particles, their properties and contribution in the final product. NanoSight can provide a number-based concentration for these nanomaterials and high-resolution size distributions for those working at the nanoscale in industrial, environmental and toxicological fields.

Ultrafine bubbles

In recent years work on ultrafine bubbles has been gathering momentum for use in industrial cleaning and water treatment, agriculture and food, and medical applications amongst others. Their relatively low concentration and small size are a challenge for many traditional nanoparticle characterization techniques whereas NTA is particularly adept at the detection and analysis of these bubbles.