Manufacturer	Malvern Panalytical
Product Series	WAVEsystem
Measurement principle	Grating-Coupled Interferometry (GCI)
Application	Biomolecular Interactions, Protein Interactions & Binding Affinity
Sample type	Suitable for Fragments, Small Molecules, Peptides, Proteins, Viruses, Cell Culture Supernatants, Serums, Cell Iysates
Temp range	15 °C – 40 °C (WAVE); 4 °C – 45 °C (WAVEdelta)
Flow Channels / Buffer	2- channel/1 buffer (WAVE); 4-channel/ 4 buffers (WAVEdelta)
Sample Capacity	2x microtiter plates (96 or 384 well, standard or deep well) or vial racks (48 positions of 1.5ml)
Information Provided	Kinetic and affnity data (ka, kd, KD)

The WAVEsystem provides rapid, label-free and high-sensitivity analysis to study real-time biological interactions providing deeper insight into the affinity and kinetics of the interaction as well as binding specificity.

Engineered around the patented Grating-Coupled Interferometry (GCI) technology, a range of analytes can be investigated in just one system to manage a broader range of samples than traditional SPR equipment. This extremely versatile technology can screen crude and complex samples involving fragment, small molecules, peptides, membrane proteins, biologics and other molecules even in crude reaction mixtures and biofluids like undiluted serum and plasma.

Large molecules with high affinity and slow dissociation can be measured simply (even native proteins in complex matrices) without purification. This is made possible by WAVEchip, a microfluidics pathway that is fully contained in single, disposable 2- or 4 channel cartridge complemented by integral no-clog technology.

With ultra-fast transition times (up to 150ms), reliable determination of off-rates of 10s-1 can be calculated for the kinetic study of small and weak binding fragments. This is aided by monitoring through the waveguide so that an increased number of binding interactions can contribute to the overall signal.

The waveRAPID (Repeated Analyte Pulses of increasing duration) method can also be used to increase throughput compared to multi-cycle kinetics, particularly in screening applications, by generating a pulsating concentration profile.

Easy-to-use software, WAVEcontrol, makes data interpretation and evaluation straight forward. The kinetic series can be evaluated in just one click, as the software automatically adjusts the data and initiates the evaluation process. Smart wizards extend the automated capabilities of the system and include: Ligand screening – a faster, flexible way to screen and characterise antibodies and Calibration-free concentration analysis (CFCA) – a reliable, quick and easy calibration-free approach to quantify active protein concentration.

Resources

Demonstration Benefits Applications FAQs

All sizes welcome. Screen, rank and characterise small analytes and fragments, including weak binders with fast dissociation rates (koff as high as 10/sec).
Kinetic profiling closer to real life. Assess drug performance in undiluted human blood serum and plasma samples.
Broadest kinetic range. Quantify binding affinities (Kd) from low pM to high uM with confident kinetic analysis.
More than buffer. Explore more solubilisation and purification conditions for membrane proteins, including viscous detergents, less common solvents, and different additives.
No clogging, regardless of the size. Analyse and characterise larger molecules (VLPs, liposomes) and aggregates (fibrils) without clogging-risk.
Tight binders welcome. Measure kinetics of high-affinity binders at low pM affinity (Kd) values.

Screening: off-rate, primary, fragment-based, small molecule campaigns.
Ligand interaction with membrane protein studies.
Pharmacology: kinetics in biofluids (blood serum, plasma) for drug potency assessment.
Biologics: characterisation and quantification of antibodies at low limits of detection in biofluids or media.
For more application notes click here

What is the Creoptix WAVE system used for?

The Creoptix WAVE system is a label-free biosensor used to study biomolecular interactions in real time, providing accurate kinetic and affinity data for protein-protein, protein-small molecule, antibody, and nucleic acid interactions.

What measurement technology does the Creoptix WAVE system use?

The WAVE system uses Grating-Coupled Interferometry (GCI), a highly sensitive optical technique that detects changes in refractive index at the sensor surface as biomolecules bind and dissociate.

How does GCI differ from SPR?

GCI offers higher sensitivity (especially for small molecules), lower baseline drift and improved stability, and more accurate kinetic measurements across a wider range of affinities. The WAVE system provides high-quality association (ka), dissociation (kd), and affinity (KD) data, enabling detailed understanding of binding mechanisms.

Is the Creoptix WAVE system suitable for fragment-based drug discovery?

Yes. Thanks to the high sensitivity of GCI, the WAVE system is particularly effective for fragment screening and weak or transient interactions that are difficult to measure with other techniques.

What are the key advantages of the Creoptix WAVE system?

Key benefits include exceptional sensitivity, stable baselines, accurate kinetic data, low sample consumption, and flexibility across a wide range of biomolecular interactions. The WAVE system comes with a temperature-controlled autosampler that fits vials, two 96- or one 384-well plates. It can analyse crude samples, harsh chemicals and particles up to 1000 nm.

Can I have a demonstration or free trial of the Creoptix WAVE?

Yes, if a system is available. Contact us for more information. We can arrange a personalised demo and test your samples within our lab facility in Sydney. Contact us or complete a request a demo form to get started.

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