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H-600 Automatic Exosome Isolation

Exodus-bio

EXODUS H-600 is an automatic, label free, highly efficient exosome isolation and purification system.
Manufacturer EXODUS
Product Series EXODUS
Measurement principle Purification
Application Exosome isolation, Exosome Tracking
Sample type Plasma, urine, saliva, cerebrospinal fluid, tears, aqueous humor, synovial fluid, tissue, cell culture medium, bacterial culture medium, cell-derived vesicle, plant, etc
Isolation principle Dual-Membrane Nanofiltration System
Sample volumes 10 μL – 250 mL
Exosome recovery volumes 100 – 1000 μL
Processing speed Max speed 200 mL/h

EXODUS H-600 is an automatic, label free, highly efficient exosome isolation and purification system. High-quality intact exosomes can be quickly and easily isolated with excellent yield and purity from various biofluids (including blood, tears, urine, and saliva) and various sample volumes.

EXODUS is based on ultrasonic nano-filtration technology. Unlike other isolation methods – like ultracentrifugation, ultrafiltration, chromatography, polymer-based precipitation, and antibody-coupled magnetic beads – EXODUS achieves clog-free ultra-fast purification of exosomes in high yield while eliminating contaminants including cell-free nucleic acids and lipoproteins which interfere with exosomal biomarker interpretation.

EXODUS has been developed using a dual-membrane nanofiltration system that integrates periodic negative pressure oscillation (NPO) and double-coupled ultrasonic harmonic oscillations (HO). The exosomes are precisely intercepted by a nanoporous membrane, while free nucleic acid and protein impurities are removed from the sample, resulting in the efficient purification and enrichment of exosomes.

The EXODUS H-600 exosome isolation system outperforms other isolation techniques in speed, purity and yield offering a more efficient method to help drive new discoveries in biomedical research and translation. Isolated exosomes can be used for characterisation, screening of disease markers, diagnosis and more.

Resources

  • Rapid isolation: Maximum isolation speed: 200 mL/h
  • High purity and high yield: Purity ~ 90%; Yield ~90%
  • Wide range of sample types (Urine, Plant, Cell culture medium, Cell-derived vesicle, Bacterial culture medium, Plasma, Saliva, Tears, Aqueous humor, Cerebrospinal fluid)
  • Label free: Only need PBS buffer
  • Drug delivery
  • Exosome therapeutics
  • Regenerative medicine
  • Early diagnosis

Publications 

NO. Title Journal
01 Exosome Detection via the Ultrafast-Isolation System: EXODUS Nature Methods
02 UPCARE: Urinary Extracellular Vesicles-Derived Prostate Cancer Assessment for Risk Evaluation Journal of Extracellular Vesicles
03 Discovering the Secret of Diseases by Integrated Tear Exosomes Analysis via Rapid-isolation System: ITEARS ACS Nano
04 Robust Acute Pancreatitis Identification and Diagnosis: RAPIDx ACS Nano
05 Interaction Network of Extracellular Vesicles Building Universal Analysis via Eye Tears: TNEBULA Science Advances
06 Sensitive Small Extracellular Vesicles Associated CircRNAs Analysis Combined with Machine Learning for Precision Identification of Gastric Cancer  

Chemical Engineering Journal

07 Quantitative Metabolic Analysis of Plasma Extracellular Vesicles for the Diagnosis of Severe Acute Pancreatitis Journal of Nanobiotechnology
08 Lipidomic Identification of Urinary Extracellular Vesicles for Non-Alcoholic Steatohepatitis Diagnosis Journal of Nanobiotechnology
09 Metabolomic Investigation of Urinary Extracellular Vesicles for Early Detection and Screening of Lung Cancer Journal of Nanobiotechnology
10 The Genetic Source Tracking of Human Urinary Exosomes PNAS
11 Identification and Detection of Plasma Extracellular Vesicles-derived Biomarkers for Esophageal Squamous Cell Carcinoma Diagnosis  

Biosensors & Bioelectronics

12 Audible Acoustic Wave Promotes EV Formation and Secretion from Adherent Cancer Cells via Mechanical Stimulation ACS Applied Materials & Interfaces
13 Identification of Circulating Extracellular Vesicle Long RNAs as Diagnostic Biomarkers for Patients with Severe Acute Pancreatitis  

Clinical and Translational Medicine

14 Prediction of Response to Chemoradiotherapy by Dynamic Changes of Circulating Exosome Levels in Patients with Esophageal Squamous Cell Carcinoma  

International Journal of Nanomedicine

15 Investigating The Proliferative Inhibition of HepG2 Cells by Exosome-like Nanovesicles Derived from Centella Asiatica Extract Through Metabolomics  

Biomedicine & Pharmacotherapy

16 Gut Subdoligranulum Variabile Ameliorates Rheumatoid Arthritis by Promoting TSG-6 Synthesis from Joint Cells Frontiers in Immunology
17 Metabolomic Analysis of Exosomal-Markers in Esophageal Squamous Cell Carcinoma Nanoscale
18 Metabolic Signatures of Tear Extracellular Vesicles Caused by Herpes Simplex Keratitis Ocular Surface
19 Sensitive Electrochemical Biosensor for Rapid Detection of sEV-MiRNA Based Turbo-Like Localized Catalytic Hairpin Assembly  

Analytica Chimica Acta

20 Isolation of Exosome Nanoparticles from Human Cerebrospinal Fluid for Proteomic Analysis ACS Applied Nano Materials
21 Human Umbilical Cord Mesenchymal Stem Cells Inhibit Liver Fibrosis via The MicroRNA-148a-5p/SLIT3 Axis International Immunopharmacology
22 Patient-derived Induced Pluripotent Stem Cells with a MERTK Mutation Exhibit Cell Junction Abnormalities and Aberrant Cellular Differentiation Potential  

World Journal of Stem Cells

23 Assessing Alzheimer’s Disease via Plasma Extracellular Vesicle–Derived mRNA Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring
24 Efficient Preparation of High-purity and Intact Mesenchymal Stem Cell–derived Extracellular Vesicles Analytical and Bioanalytical Chemistry
25 Plasma-derived Exosomal miR-25-3p and miR-23b-3p as Predictors of Response to Chemoradiotherapy in Esophageal Squamous Cell Carcinoma Technology in Cancer Research & Treatment
26 Comparative Investigation of Exosome Extraction from rat bone Marrow Mesenchymal Stem Cells Using Three Different Methodologies  

Electrorhoresis

27 Isolation of Small Extracellular Vesicles from a Drop of Plasma via EXODUS and Their Fingerprint Proteomics Profiling by MALDI-TOF MS  

Biosensors & Bioelectronics: X

28 Application of EXODUS System Combined with Allosteric DNA Nanoswitches in the Detection of miR-107 Among Plasma Exosomes of Parkinson′s Disease Patients  

Chinese Journal of Preventive Medicine

What is the EXODUS H-600?

The EXODUS H-600 is a fully automated, label-free exosome isolation and purification system designed for research laboratories. It uses proprietary ultrasonic nano-filtration technology to rapidly isolate high-purity, high-yield extracellular vesicles (EVs) and exosomes from a wide range of sample types.

What sample types can be processed?
The H-600 can process plasma, serum, urine, saliva, tears, cerebrospinal fluid, synovial fluid, cell culture media, bacterial culture media, tissue lysates, plant extracts, and other biological samples.

What sample volumes can the H-600 handle?

The system can process sample volumes from as little as 10 μL up to 250 mL in a single workflow. Processing speed is 200 mL/h.

How does the EXODUS technology work?

EXODUS combines Negative Pressure Oscillation (NPO) and Harmonic Oscillation (HO) within a dual-membrane nanofiltration system. This prevents membrane fouling while efficiently removing proteins, nucleic acids, and other contaminants, resulting in highly purified exosomes.

How does the H-600 compare with ultracentrifugation?
The H-600 offers significantly faster processing, reduced hands-on time, higher reproducibility, and excellent exosome purity and recovery without requiring lengthy centrifugation protocols.

Does the H-600 require antibodies or precipitation reagents?

No. The system is completely label-free and typically requires only PBS buffer, eliminating the need for expensive antibodies or precipitation chemicals.

What applications is the H-600 suitable for?

Applications include biomarker discovery, liquid biopsy research, exosome characterisation, drug delivery studies, regenerative medicine research, omics workflows, and extracellular vesicle therapeutic development.

Is the system easy to operate?

Yes. The H-600 features a 10.4-inch touchscreen interface with automated workflows, allowing users to process samples with minimal training and hands-on time.

Can I have a demonstration or free trial of the Exodus H-600?

Yes, if a system is available. Contact us for a booking. We can arrange a personalised demo and test your samples within our lab facility in Sydney. Onsite free trials are also available. Contact us or complete a request a demo form to get started.

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