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X-Clarity

Logos Biosystems (Aligned Genetics, Inc.)

The X-CLARITY is an all-in-one system and ready-to-use reagents for simple, rapid and efficient tissue clearing to allow for 3D analysis of the anatomical structure of tissues.
Manufacturer Logos Biosystems
Product Series X-CLARITY
Measurement principle Microscopy – Cell image analysis
Application Fluorescence Imaging, Live cell image analysis
Type Tissue clearing system
Throughput Up to 768 samples
Temperature RT to 60°C

The X-CLARITY is an all-in-one system with ready-to-use reagents used to standardise, simplify and accelerate the tissue clearing process – the transformation of biological tissue into a transparent state to light.

X-CLARITY has a unique design that accelerates the removal of lipids from tissues in a highly efficient manner. It enables ultrafast (in just 6 hours!) and reproducible clearing results to aid subsequent whole tissue 3D and fluorescence imaging.

Preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence imaging downstream.

Precise temperature control

  • Active buffer cooling and heating capacity
  • Sensitive and accurate temperature sensor

Uniform electric field

  • Platinum-plated electrodes
  • Constant current and constant voltage modes

Compatible with multiple tissue types and sizes

  • Electrophoretic and passive clearing
  • Holders of various sizes available

User-friendly setup

  • Simple touchscreen interface
  • Ready-to-use clearing solution

Resources

X-CLARITY Tissue Clearing System:

  • Dramatically reduces tissue clearing time. A whole mouse brain is cleared in just 6 hours.
  • Successfully produces transparent tissues that demonstrate the efficient penetration of and labeling with macromolecules such as antibodies or oligonucleotides.
  • Greatly simplifies the tissue clearing process. Specially designed consumables make tissue clearing even more straightforward and trouble-free.

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