Malvern Zetasizer APS

The Malvern Zetasizer APS is a Dynamic Light Scattering system that incorporates Automated Plate Sampling to determine protein conformation and stability over a wide range of developmental conditions.

Malvern Zetasizer APS

The Malvern Zetasizer APS is a Dynamic Light Scattering system that incorporates Automated Plate Sampling to determine protein conformation and stability over a wide range of developmental conditions.

Manufacturer Malvern
Product SeriesMalvern Zetasizer
Measurement principleDynamic Light Scattering
ApplicationProtein Interactions & Binding Affinity
Sample typeProteins
Particle size range0.3nm – 2.0µm
Minimum sample volume20µL
AccuracyBetter than +/-2%
Sensitivity0.1mg/mL 15kDa protein (Lysozyme)
Aggregation temperature(melting point) range: 2ºC - 90°C
Sample storage4 - 40°C

Product Overview

The Malvern Zetasizer APS uses Dynamic Light Scattering (DLS) to determine protein conformation and stability. It incorporates an Automated Plate Sampling system for the analysis of samples in 96- or 384-well plates.

A recoverable 20µL sample volume is transferred from each plate well to a precision quartz flow cell for measurement. The flow cell is temperature controlled and its optical clarity ensures accurate and precise results. After measurement the sample is returned to its well.

Dynamic Light Scattering (DLS) determines size by measuring the Brownian movement of particles or molecules in a solvent or buffer. This diffusion speed is converted into a size distribution using the Stokes-Einstein relationship.

Zetasizers are the most widely used systems for Nano technology measurements for colloids, protein size and molecular weight. The Series includes a range of models which enables analysts to select the best system for their applications and budget.

  • Benefits
  • Applications

Benefits

  • Light scattering is not measured through the plate which means that any plate can be used and that transparency or scratches will not affect the result.
  • Light scattering is very sensitive to the presence of aggregates, so this is an ideal technique to determine the early stages of instability
  • Dual temperature control enables the samples to be kept at a low temperature to preserve the protein before and after measurement.
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Applications

  • Investigation of proteins under a large number of environmental conditions.
  • Determine protein behavior in a buffer and whether aggregates are present.
  • Screening for suitable crystallization buffers.
  • Measurement of aggregation temperature (melting point) to predict stability.
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