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LUNA Automated Brightfield Cell Counter

Logos Biosystems (Aligned Genetics, Inc.)

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The LUNA III automated cell counter is designed for cost efficient, fast and accurate brightfield cell counting.
Manufacturer Logos Biosystems
Product Series LUNA
Measurement principle Microscopy – Cell image analysis
Application Live cell image analysis
Cell size range 3 – 60 µm
Optics Brightfield

LUNA-III Automated Cell Counter  is an affordable, fast and accurate brightfield cell counter designed to enhance your lab’s productivity and precision. Building on the proven success of the LUNA-II, the LUNA-III Automated Cell Counter offers an array of advanced features.

Using Machine Learning trained algorithms LUNA-III Automated Cell Counter can  more effectively identify a wide range of cell types and accurately identify single cells in aggregated samples. In this way LUNA III addresses the common limitations of dedicated bright field cell counters, which typically use less sophisticated cell recognition algorithms that struggle to accurately identify diverse cell morphologies or assess cell viability.

The LUNA III is a stand-alone, compact instrument that integrates precision optics with fast and accurate autofocus capability to optimise light levels and to capture high-resolution images while image analysis software enables accurate cell count and viability data. Users can create custom, cell-specific cell counting protocols or simply use the default counting protocol suitable for most cell lines. Live cells are tagged with green circles and dead cells are tagged with red circles, making it easy to verify the accuracy of each count. This compact cell counter sits comfortably in a cell culture hood or on a lab benchtop to fit seamlessly into user workflows.

Ideal for labs on a budget, the LUNA III automated cell counter can count samples with or without staining. Users may select to stain cells with trypan blue or Erythrosin B for viability data. It accurately detects total/live/dead cells at concentrations ranging from 5×104 to 1×107 cells/ml and cell sizes between 3 and 60µm.

The LUNA Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. LUNA disposable slides offer further convenience and maintain the highest standard of cell counting accuracy with the added benefit of no mess or cleanup.

Resources

  • Most affordable, fully automated cell counting and cell viability analysis
  • Machine learning algorithms to accurately identify single cells in aggregated samples
  • Autofocus technology for fast and reliable cell counting
  • Various histograms and cell-size gating
  • Unmatched counting accuracy and speed
  • Built-in computer and printer
  • Cost efficient reusable slides offer accurate cell counting without the associated subjectivity and time.
  • Disposable cell counting slides for no mess or cleanup.

Where the LUNA-III and LUNA-II has been cited

https://logosbio.com/citations/?citations_category=luna-ii

  • Effect of Atmospheric Aging on Soot Particle Toxicity in Lung Cell Models at the Air–Liquid Interface: Differential Toxicological Impacts of Biogenic and Anthropogenic Secondary Organic Aerosols (SOAs).
    2022. Offer S, Hartner E, Di Bucchianico S, Bisig C, Bauer S, Pantzke J, … & Zimmermann R. Environmental health perspectives, 130(2), 027003.
  • Dry powders containing chitosan-based nanocapsules for pulmonary administration: Adjustment of spray-drying process and in vitro evaluation in A549 cells.
    2022. Fernández-Paz E, Fernández-Paz C, Barrios-Esteban S, Santalices I, Csaba N, Remuñán-López C. Powder Technology, 399, 117149.
  • A mucin-responsive hybrid two-component system controls Bacteroides thetaiotaomicron colonization and gut homeostasis.
    2022. Lee JH, Kwon SJ, Han JY, Cho SH, Cho YJ, Park JH. Journal of Microbiology, 60(2), 215-223.
  • The role of YAP1 in small cell lung cancer.
    2022. Saito H, Tenjin Y, Yamada T, Kudoh S, Kudo N, Sanada M, … & Ito T. Human Cell, 35(2), 628-638.
  • Microalgal feeding preference of Mytilus coruscus and its effects on fatty acid composition and microbes of the digestive gland.
    2022. Zhang XL, Li SY, He JY, Wu YH, Gu ZQ, Fan MH, … & Yan XJ. Aquaculture Reports, 23, 101024.
  • Combined Approach to Leukemic Differentiation Using Transcription Factor PU. 1-Enhancing Agents.
    2022. Bašová P, Paszeková H, Minařík L, Dluhošová M, Burda P, Stopka T. International journal of molecular sciences, 23(12), 6729.
  • Identification of four novel T cell autoantigens and personal autoreactive profiles in multiple sclerosis.
    2022. Bronge M, Högelin KA, Thomas OG, Ruhrmann S, Carvalho-Queiroz C, Nilsson OB, … & Grönlund H. Science advances, 8(17), eabn1823.
  • Multiplexed miRNA detection based on target-triggered transcription of multicolor fluorogenic RNA aptamers.
    2022. Park Y, Yoon J, Lee J, Lee S, Park HG. Biosensors and Bioelectronics, 204, 114071.
  • Recombinant Lloviu virus as a tool to study viral replication and host responses.
    2022. Hume AJ, Heiden B, Olejnik J, Suder EL., Ross S, Scoon WA, … & Mühlberger E. PLoS pathogens, 18(2), e1010268.
  • Protocol for chronic hepatitis B virus infection mouse model development by patient-derived orthotopic xenografts.
    2022. Nagornykh AM, Tyumentseva MA, Tyumentsev AI, Akimkin VG. Plos one, 17(2), e0264266.
  • Styryl Dyes with N-Methylpiperazine and N-Phenylpiperazine Functionality: AT-DNA and G-quadruplex Binding Ligands and Theranostic Agents.
    2022. Zonjić I, Stojković MR, Crnolatac I, Paić AT, Pšeničnik S, Vasilev A, … & Tumir LM. Bioorganic Chemistry, 105999.
  • Wenyang Huazhuo Tongluo formula alleviates pulmonary vascular injury and downregulates HIF-1α in bleomycin-induced systemic sclerosis mouse model.
    2022. Li K, Wang Q, Lv Q, Guo K, Han L, Duan P, … & Bian H. BMC Complementary Medicine and Therapies, 22(1), 1-12.
  • Polychromatic Quantum Dot Array to Compose a Community Signal Ensemble for Multiplexed miRNA Detection.
    2022. Nam TW, Park Y, Jung YS, Park HG. ACS nano.
  • Inactivated whole-virion vaccine BBV152/Covaxin elicits robust cellular immune memory to SARS-CoV-2 and variants of concern.
    2022. Vikkurthi R, Ansari A, Pai AR, Jha SN, Sachan S, Pandit S, … & Gupta N. Nature Microbiology, 1-12.
  • Biocompatible fluorescent silk fibroin bioink for digital light processing 3D printing.
    2022. Lee YJ, Lee JS, Ajiteru O, Lee OJ, Lee JS, Lee H, … & Park CH. International Journal of Biological Macromolecules.
  • Design, synthesis, and biological evaluation of novel pyrazolo [3, 4-d] pyrimidine derivatives as potent PLK4 inhibitors for the treatment of TRIM37-amplified breast cancer.
    2022. Sun Y, Sun Y, Wang L, Wu T, Yin W, Wang J, … & Cheng M. European Journal of Medicinal Chemistry, 114424.
  • Nonfreezing Low Temperature Maintains the Viability of Menstrual Blood–Derived Endometrial Stem Cells Under Oxygen–Glucose Deprivation Through the Sustained Release of Autophagy-Produced Energy.
    2022. Chen T, Zhang S, Jin H, Fu X, Shang L, Lu Y, … & Lin J. Cell transplantation, 31, 09636897221086971.
  • Rixosomal RNA degradation contributes to silencing of Polycomb target genes.
    2022. Zhou H, Stein CB, Shafiq TA, Shipkovenska G, Kalocsay M, Paulo JA, … & Moazed D. Nature, 604(7904), 167-174.
  • A hydroxypropyl methylcellulose plaque assay for human respiratory syncytial virus.
    2022. Takumi-Tanimukai Y, Yamamoto S, Ogasawara N, Nakabayashi S, Mizuta K, Yamamoto K, … & Yokota SI. Journal of Virological Methods, 304, 114528.
  • EPHB2 expression is associated with intestinal phenotype of gastric cancer and indicates better prognosis by suppressing gastric cancer migration.
    2022. Kim H, Myung JK, Paik SS, Kim H, Park H, Kim YJ, … & Jang B. American journal of cancer research, 12(3), 1295.

What types of cells can the LUNA-III count?

The LUNA-III is designed for counting a wide range of mammalian cells, including suspension cells, primary cells, stem cells, immune cells, and cultured cell lines. Its advanced algorithms improve recognition of different cell types and aggregated samples.

How does the LUNA-III determine cell viability?

The LUNA-III performs viability analysis using Trypan Blue or Erythrosin B staining. It automatically distinguishes live and dead cells, providing cell concentration, viability percentage, and size distribution within seconds.

What advantages does the LUNA-III offer over manual haemocytometer counting?

The LUNA-III delivers faster, more reproducible results while eliminating operator subjectivity. Automated image analysis reduces counting errors, improves consistency between users, and significantly increases laboratory productivity. Advanced algorithms enable the LUNA-III to identify individual cells within clustered or aggregated samples more accurately than conventional image-based counting methods.

Does the LUNA-III require disposable slides?

No. Users can choose between disposable counting slides for convenience or reusable glass slides for lower running costs and reduced plastic waste.

Can I review and reanalyse previous results?

Yes. The LUNA-III includes a unique reanalysis feature that allows users to revisit stored images, adjust counting parameters, and refine results without preparing a new sample.

How are data and reports managed?

Results can be stored internally, transferred via USB, or accessed through network connectivity. Data can be exported as PDF reports, CSV files, and image files for documentation and regulatory compliance.

What makes the LUNA-III suitable for busy laboratories?

The compact footprint, touchscreen interface, autofocus capability, customizable protocols, rapid analysis, and automated reporting make the LUNA-III ideal for research, bioprocessing, cell therapy, and quality control laboratories.

Can I have a demonstration or free trial use or hire the LUNA III?

Yes, if a system is available. Contact us for a booking. We can book a personalised demo to show you how the system works and arrange for your samples to be tested within our lab facility, based in Sydney. We also arrange onsite free trials. It is very quick and easy to set up. To set up a free trial is easy – simply contact us or send us a message by completing the request a demo.

LUNA-BX7 automated cell counter offers everything you need for high-performance brightfield cell counting at an affordable price.
Manufacturer Logos Biosystems
Product Series LUNA
Measurement principle Microscopy – Cell image analysis
Application Live cell image analysis
Cell diameter range Detectable: 1-90 µm, optimal: 3-60 µm
Optics Brightfield
Multi chamber slides 1-, 2-, 3-, and 8-channel configurations plus reusable slide
GMP compliance (QC/Bioprocessing) CountWire CFR 21, Part 11 compliance

LUNA-BX7 Automated cell counter offers high-performance cell counting at an affordable price. The LUNA-BX7 retains the core features of the LUNA-FX7 while focusing solely on brightfield optics.

High-resolution imaging and expanded concentration volume

Equipped with advanced algorithms and machine-learning capabilities, it can count diverse and more complex cell lines at a higher throughput while enabling accurate, reliable, and reproducible results. The small footprint makes it ideal as a benchtop cell counter for busy labs with multiple users, a high volume of daily cell counts, or for bioprocessing and GMP compliance.

Higher throughput & multiple slide options

LUNA-BX7 offers 1-, 2-, 3-, and 8-channel slide configurations, similar to the LUNA-FX7.The reusable slide provides an eco-friendly and budget-friendly option, while the disposable slides provide peace of mind for cell counts where cross-contamination is a factor. User-friendly software makes it easy to switch between chambers. This means unused chambers in a multi-chamber slide can be used, eliminating waste.

Quality Control, Bioprocessing & 21 CFR, Part 11 

LUNA-BX7 provides QC software (IQ/OQ protocol) for daily checks and maintaining accuracy, offering Validation Slides for brightfield cell counting to ensure the highest standards in your cell-counting workflow. Bioprocessing capabilities are available providing growth rates and trend charts to track production and accurately forecast timelines, allowing users to monitor, record, and analyse different bioprocessing activities individually. LUNA-BX7 features the optional CountWire software for the ultimate in CFR 21, Part 11 compliance.

LUNA-BX7 can be upgraded to LUNA-FX7 to add two-channel fluorescence optics if needs change.

Resources

Affordability. A quality, high-throughput automated cell counter at an affordable price point, offering high performance and upgraded features, makes it a wise long-term investment.

LUNA-FX7 platform. The LUNA-BX7 was developed using the same advanced software, machine-learning capabilities, state-of-the-art precision optics, and hardware used to create the trusted, award-winning LUNA-FX7.

Multiple slide formats. Provides increased cell concentration volume and improves accuracy by offering up to 1 – 47 fields of view per count.

Disposable slide option. A key consideration for labs with multiple users with different needs and protocols that may require disposable slides to protect the integrity of sensitive research, GMP requirements, or data intended for publications.

LUNA Reusable Slide. With the same quality and sensitivity as disposable slides, it offers both an eco-friendly and budget-friendly option.

Small footprint. The LUNA-BX7 is benchtop-ready, leaving plenty of room to work in a shared-space environment.

Upgrade to fluorescence. The LUNA-BX7 to LUNA-FX7 upgrade pathway adds long-term value, providing an option for expanding research capacity in the future.

What is the LUNA-BX7?

The LUNA-BX7 is a high-throughput automated cell counter for labs that process large numbers of samples. It is ideal for bioprocessing, cell therapy, immunology, vaccine development, and cell culture laboratories seeking faster and more consistent cell analysis.

How does the LUNA-BX7 improve laboratory productivity?

The LUNA-BX7 can process up to seven samples in a single run. This reduces operator intervention, increases throughput, and helps standardise cell counting workflows across multiple users.

What types of cells can the LUNA-BX7 analyse?

The system is suitable for a broad range of mammalian cells, including suspension cultures, primary cells, stem cells, hybridomas, CHO cells, HEK293 cells, T cells, and other immune cell populations commonly used in research and biomanufacturing.

What information does the LUNA-BX7 provide?

The LUNA-BX7 automatically measures total cell count, viable cell count, cell concentration, viability percentage, and cell size. Results are displayed in an easy-to-review format and can be exported for further analysis or record keeping.

How does the LUNA-BX7 determine cell viability?

The instrument uses Trypan Blue exclusion staining to differentiate live and dead cells. Advanced image analysis algorithms automatically identify and classify cells, providing accurate and reproducible viability measurements without subjective manual interpretation.

Is the LUNA-BX7 suitable for GMP and regulated environments?

Yes. The system supports standardised workflows, automated data management, and reduced operator variability, making it well suited for GMP-regulated laboratories and biomanufacturing environments where consistency and traceability are critical.

Can I have a demonstration or free trial use or hire the LUNA-BX7?

Yes, if a system is available. Contact us for a booking. We can book a personalised demo to show you how the system works and arrange for your samples to be tested within our lab facility, based in Sydney. We also arrange onsite free trials. It is very quick and easy to set up. To set up a free trial is easy – simply contact us or send us a message by completing the request a demo.

Variant comparison

LUNA Automated Brightfield Cell Counter III

Cell counter that is versatile and affordable
Brightfield Optics
Liquid lens
Cell diameter range: 3~60 μm

LUNA Automated Brightfield Cell Counter BX7

Automated, unmatched accuracy for higher throughput
Brightfield Optics
Advanced microscopy lens
Cell diameter range: 1-90 μm

LUNA III LUNA BX7
Cell counter that is versatile and affordable Cell counter for higher throughput and/or regulatory needs
Optics
Brightfield Brightfield
Auto Focus / Manual Focus Auto Focus / Manual Focus
Cell types
Stable cell lines Stable cell lines
Compatible Dyes
Trypan blue
Erythrosin B
Trypan blue
Erythrosin B
Methylene Blue
Liquid lens Advanced microscopy lens
Cell diameter range
3~60 μm 1-90 μm
Sample loading volume
10 μL-50 μL (depends on slide type) 10 μL-50 μL (depends on slide type)
Maximum analysis volume
0.5 μL-5.1 μL (depends on slide type) 0.5 μL-5.1 μL (depends on slide type)
Cell Concentration Range
5×104~1×107 cells/mL Detectable: 1 x 10⁴ – 5 x 10⁷ cells/mL
Slide Options: LUNA™ 1-Channel Slide; LUNA™ 3-Channel Slide; LUNA™ 8-Channel Slide; LUNA™ Cell Counting Slide; LUNA™ Reusable Slide
✓ (LUNA™ Cell Counting Slide; LUNA™ Reusable Slide only) ✓ (all slide options)
Onboard storage
10 GB 250GB/1TB
21 CFR Part 11 Compliance

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