How does your phase imaging measure up?

17 May, 2018 | Newsletters
How does your phase imaging measure up?

Many phase contrast imaging and analysis techniques claim to be quantitative, but may use indirect or proxy measurements to define and quantify cells.

Quantitative Phase Imaging (QPI) generates high contrast, high quality images from which direct measures of morphology and motility can be extracted, allowing comprehensive analysis at both population and individual cell level.

Achieve a greater level of scrutiny with QPI

Quantitative Analysis
• High resolution imaging defines EVERY cell to give a true measure of cell count.

• DIRECT measurement of cell metrics. Suitable for heterogeneous populations where smaller sub populations can be differentiated.

• Computational technique. Calculates the extent of phase shift allowing thickness dependent measures to be determined for each cell
e.g. cell volume, dry cell mass and sphericity.

Phase Contrast
Quantitative Analysis
• Insufficient resolution to define each cell. Confluence used to ESTIMATE cell count.

• Analysis based on INDIRECT population level metrics, i.e.confluency, which characterises the cell population as homogeneous.

• Optical technique.
Unable to assess the extent of phase shift so individual cell parameters cannot be determined.

QPI – made to measure imaging
Is your phase imaging technique truly quantitative?
Compare the differences in outcomes between QPI and Phase Contrast imaging

Watch the short video to see how QPI metrics can enhance your data analysis

“Characterising live cell behaviour: Traditional label-free and quantitative phase imaging approaches”.

Click here for your copy of the recent paper

The ATA Scientific Encouragement Award aims to provide young scientists with financial assistance to further their education and attend scientific meetings and conferences.

Simply outline in 500 words or less your answer to the question on our website. The participant who submits the most amusing or imaginative entry will receive the A$1500 award. There are also two runner up awards of $600 each.

Competition closing date 30 April 2018. Conditions apply.

Click here to enter our award

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