Adsorption and aggregation of monoclonal antibodies at silicone oil-water interfaces
08 Jul, 2021 | Newsletters
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Protein aggregation is one of the major challenges in the development of protein-based drugs like monoclonal antibody (mAb) therapies. Being surface active, MAbs tend to adsorb to the surfaces of syringes, usually coated with a lubricant like silicone oil, which is an important source of aggregation.
This webinar discusses the adsorption behaviour of two monoclonal antibodies with different propensities to aggregate at these interfaces. The surface activity was studied through measurements of oil-water interfacial tension, surface mass adsorption (using Quartz Crystal Microbalance, QCM-D) and interfacial rheology. The efficacy of surfactants such as polysorbates and poloxamers, typically added to stabilise mAb formulations, was also investigated.
What you will learn: Why is aggregation a challenge in monoclonal antibody drug formulations in the biopharmaceutical industry? How does the adsorption of monoclonal antibodies to surfaces and interfaces lead to aggregation? How are surfactants, added to drug formulations, effective in mitigating aggregation?
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