Spectroscopy & chromatography
The two major areas of the analytical sciences are Spectroscopy and Chromatography. Spectroscopy covers a wide range of techniques for chemical analysis at the atomic and molecular level. Chromatography is a general term for techniques that separate, identify and quantify compounds.
Click here to contact usSpectroscopy is the interactions of matter with radiation particularly electromagnetic region such as UV visible and infrared light. The range of light analysis techniques includes absorbance, reflection, emission, scattering and refractive index. The physical quantity measured is either of energy absorbed or energy produced.
Absorption Spectroscopy includes atomic absorption and molecular techniques, such as infrared and ultraviolet-visible. Emission Spectroscopy includes fluorescence and one of the main examples of Scattering Spectroscopy, is Raman Spectroscopy. Other commonly used spectroscopic analysis techniques are Circular Dichroism , Dual Polarisation Interferometry and Laser Spectroscopy.
Reaction Kinetics are usually detected and recorded by spectroscopic techniques such as UV absorbance, fluorescence or circular dichroism. One of the main rapid kinetics techniques is stopped-flow, commonly used with fluorescence detection due to its high sensitivity.
High pressure liquid chromatography. The sample to be analysed is dissolved in a liquid which is pumped through a column packed with particles. As the analyte traverses the length of the column it is slowed by specific chemical or physical interactions with the particles. When the analyte emerges from the column it passes through a detector which shows a peak with a retention time characteristic for that analyte. Traditional HPLC is performed with columns packed with particles usually around 5 μm diameter. Smaller particles (<2 μm) provide more surface area with significant increases in resolution, speed, and sensitivity.
Gel permeation chromatography (GPC) separates particles on the basis of size. It is used for measuring the molecular weight of large molecules such as proteins or polymers.
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