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QUANTOM Tx Microbial Cell Counter

Logos Biosystems (Aligned Genetics, Inc.)

QUANTOM Tx for rapid and accurate automated single bacterial cell counting in less than 30 seconds.
Manufacturer Logos Biosystems
Product Series QUANTOM
Measurement principle Automated Fluorescence Microscopy
Application Live cell image analysis
Sample type Microbial, Bacterial Cell
Cell size range ~0.3-50 µm
Excitation/Emission spectra Ex 470/30 nm, Em 530/50 nm

The QUANTOM Tx Microbial Cell Counter is an image-based, automated cell counter that can identify and count individual bacterial cells in minutes, not days. With no culturing needed, QUANTOM Tx automatically focuses, captures and analyses multiple images of fluorescence-stained cells to detect bacterial cells with high sensitivity and accuracy. The novel bacteria-cell detection software is able to detect individual cells despite genome size, morphology, cell size, or motility, and even in the tightest of clusters.

Utilising two different stains, the QUANTOM Tx can count total cells or viable cells. Stained cells are mixed with QUANTOM Cell Loading Buffer I, loaded into QUANTOM M50 Cell Counting Slides, and spun in the QUANTOM Centrifuge to immobilise and evenly distribute the cells along a single focal plane to ensure accurate cell detection. Counting results and images can be viewed and saved immediately after the count.

Resources

RAPID

  • No culturing required – from sample to result in minutes

ACCURATE

  • Objective results – no user-to-user variability, no estimates based on CFU’s (Colony Forming Units) or turbidity

SINGLE BACTERIUM DETECTION

  • Advanced cell counting software will count down to single bacterial cell regardless of shape, size or arrangement
  • Choose either total or viable only cell counts – using specific fluorescence stains

EASY TO USE

  • Easy to follow protocol and autofocus. Large touchscreen interface with intuitive software

Bacteria quantification is a one of the most fundamental measurements in a number of fields, including healthcare, agriculture, food production, and industry. From the need to accurately quantify live ‎Lactobacillus casei in probiotics, heat-killed Vibrio cholera in a vaccine, sulfur oxidising bacteria for waste treatment, general bacterial levels to determine soil contamination, or just your standard cell suspension in a laboratory, bacterial cell counting is a basic but significant step.

QUANTOM Tx™ Microbial Cell Counter for rapid enumeration: comparison with existing methods

Implementing New Standards in Metagenomics and the Extreme Microbiome Project

Are Mammalian cell counters good for counting microbes like bacteria?

Standard mammalian cell counters are not suited to counting microbes given the microbe size and apparent motility. Although smaller and simpler than their eukaryotic counterparts, bacteria are an incredibly diverse group of organisms that come in multiple shapes, sizes, and arrangements. The QUANTOM Tx Microbial Cell Counter is an image-based, automated cell counter that can detect individual bacterial cells in mere minutes. Its novel bacteria-optimised cell detection software can count individual bacterial cells in even the tightest clusters.

How does the QUANTOM Tx count cells?

The QUANTOM Tx counts fluorescence-stained microbial cells through automated fluorescence imaging and analysis. The QUANTOM Centrifuge is crucial for distributing cells along a single plane for accurate counting with the QUANTOM Tx.

What kinds of cells does the QUANTOM Tx count?

The QUANTOM Tx is optimised to count bacterial cells, but may be able to count other cell types.

How long does the QUANTOM Tx take to count?

It takes ~30 seconds per sample when capturing and analysing 10 images.

What size cells can the QUANTOM Tx detect?

Cells ~0.3-50 μm can be counted.

Can I use different slides with the QUANTOM Tx?

No. Bacteria are small and need to be loaded into special chambers for even distribution. QUANTOM M50 Cell Counting Slides have been designed specifically for microbes for use with the QUANTOM Tx and the QUANTOM Centrifuge.

How often do I have to calibrate the QUANTOM Tx?

The QUANTOM Tx comes pre-calibrated so it does not need to be calibrated again before use. Calibrate the instrument after each software update.

Can I have a demonstration or free trial use or hire the Quantom Tx?

Yes, if a system is available. Contact us for a booking. We can book a personalised demo to show you how the system works and arrange for your samples to be tested within our lab facility, based in Sydney. We also arrange onsite free trials. It is very quick and easy to set up. To set up a free trial is easy – simply contact us or send us a message by completing the request a demo.

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