LUNA Fluorescence Cell Counter

Logos Biosystems LUNA-FL with dual fluorescence and brightfield optics accurately determines cell count, cell viability and GFP transfection efficiency without being limited by cell type or size.

Manufacturer	Logos Biosystems
Product Series	LUNA
Measurement principle	Microscopy – Cell image analysis
Application	Fluorescence Imaging, Live cell image analysis
Cell counting time	30 sec
Cell size range	1 to 90 μm
Ex/Em wavelength	470±20 nm/ 530±25 nm, 600 nm(LP)

LUNA-FL enables automated cell counting and cell viability analysis. This stand-alone, compact instrument combines brightfield and dual fluorescence optics to enable four counting modes: Brightfield cell counting; fluorescence cell counting; yeast cell counting and GFP transfection assays.

Integrated dual fluorescence microscope optics, allow primary cells such as PBMCs, splenocytes, neutrophils and stem cells to be distinguished from undesirable debris, usually confused for cells with conventional cell counters.

Whether counting live and dead cells with trypan blue staining or performing GFP transfection analysis using fluorescently stained cells, LUNA-FL provides the most sensitive cell analysis results. A histogram displays live and dead cell populations allowing counted cells to be gated based on cell size information.

Designed for cost-efficient and accurate cell counting the LUNA automated cell counter is compatible with both reusable and disposable cell counting slides to maintain the highest standard of cell counting accuracy.

The LUNA family of automated counters has been awarded a Diamond seal of quality from SelectScience in recognition of outstanding feedback received from scientists globally – the first diamond 6 winner since the awards were launched in 2017!

TWO COUNTERS IN ONE
LUNA-FL can count in both brightfield and fluorescence mode. Dye exclusion based cell counting with brightfield optics.

COMPATIBLE WITH MOST CELLS
Advanced imaging software and GFP and RFP fluorescence optics detect fluorescence stained cells with high sensitivity. Optimised for primary cells, stem cells, PBMCs, Splenocytes, etc.

GFP TRANSFECTION ANALYSIS
High-resolution images are captured and analysed to detect GFP-positive and -negative cells.

MAXIMUM 30 SECONDS
Fluorescence: 30 s = 3 images captured & analyzed; Brightfield: 10 s = 1 image captured & analysed.

ADVANCED CLUSTER ANALYSIS
An advanced declustering algorithm detects individual cells in clusters.

USER-FRIENDLY INTERFACE
Customise and save protocols, verify results immediately onboard, use the dilution calculator, and gate cells on histograms with a few taps on the LUNA-FL touchscreen.

SIMPLE RECORDKEEPING
Print data with an external printer or export high-resolution images, data and full data reports via USB.

THE LOWEST COST, THE GREATEST CONVENIENCE
Perfect for labs on a budget, the LUNA works with reusable slides, delivering the affordability of manual cell counting without the associated time, subjectivity and user-to-user variability. The disposable LUNA Cell Counting Slides offer accurate cell counting with no mess or cleanup. PhotonSlides are made with optical quality materials with ultra-low autofluorescence to allow for optimal fluorescence detection.

Count cell suspensions with cell sizes ranging from 1 to 90 μm. Applications include Primary cells (Splenocyte, Thymocyte, PBMC, Monocyte, Lymphocyte, Neuronal cell, Keratinocyte, Epithelial cell, Dendritic cell, etc), Cancer cells, Stem cells, Adipocytes, Sperm cells, Somatic cells in milk, Algae, Yeasts and more.

Cell Lines validated
On the LUNA-FL Automated cell counter
Cell Type	Animal	Organ
PBMC	Mouse	Blood
Splenocyte	Mouse	Spleen
Thymocyte	Mouse	Thymus
A431	Human	Skin
CHO-M1WT2	Chinese Hamster	Ovary
CHSE	Fish	Embryo
COLO-205	Human	Colon
Cos-7	Human	Kidney
HEK-293	Human	Kidney
HeLa	Human	Cervix
HepG2	Human	Liver
HL-60	Human	Blood
J774A.1	Mouse	Blood
Jurkat	Human	Blood
MCF7	Human	Breast
MRC-5	Human	Lung
NIH/3T3	Mouse	Embryo

What is the LUNA-FL used for?
The LUNA-FL is an automated fluorescence cell counter designed for accurate cell counting, viability analysis, and fluorescence-based assays such as apoptosis and transfection efficiency.

How does the LUNA-FL measure cell viability?
It uses fluorescence-based dyes such as AO/PI (acridine orange/propidium iodide) to distinguish live, dead, and in some cases apoptotic cells with higher accuracy than brightfield staining methods.

What is the advantage of fluorescence counting over traditional Trypan Blue methods?
Fluorescence detection improves accuracy by reducing background interference and better distinguishing live and dead cells, particularly in samples with debris or low contrast.

What types of cells can be analysed?
The LUNA-FL can analyse a wide range of mammalian cells including suspension cells, adherent cells (in suspension after detachment), stem cells, and immune cells.

What applications is the LUNA-FL commonly used for?
It is widely used in cell therapy research, bioprocess development, transfection studies, apoptosis assays, and general cell culture monitoring.

Does the LUNA-FL provide additional cellular analysis beyond viability?
Yes. Depending on the assay kit used, it can provide information such as live/dead ratios, apoptotic populations, and fluorescence intensity-based measurements.

How fast is an analysis run?
Most measurements are completed within seconds, enabling rapid processing of multiple samples with minimal hands-on time.

How does it improve reproducibility compared to manual methods?
Automated imaging and fluorescence-based classification reduce user subjectivity, improving consistency between operators and across experiments.

Can I have a demonstration or free trial use or hire the LUNA-FL?
Yes, if a system is available. Contact us for a booking. We can book a personalised demo to show you how the system works and arrange for your samples to be tested within our lab facility, based in Sydney. We also arrange onsite free trials. It is very quick and easy to set up. To set up a free trial is easy – simply contact us or send us a message by completing the request a demo.

The LUNA-STEM takes adipose-derived stem cell and SVF samples to count live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.

Manufacturer	Logos Biosystems
Product Series	LUNA
Measurement principle	Microscopy – Cell image analysis
Application	Fluorescence Imaging, Live cell image analysis
Sample volume	10 μl
Cell counting time	30 sec
Cell size range	1 to 90 μm
Ex/Em wavelength	470±20 nm/ 530±25 nm, 600 nm(LP)

The LUNA-STEM is an automated, compact cell counting and viability analysis system that combines dual fluorescence optics and image analysis software. Its interactive touchscreen makes accurate cell counting a simple experience.

Using Acridine Orange (AO) and Propidium Iodide (PI) to stain live and dead cells, respectively, the LUNA-STEM™ provides highly sensitive and accurate results for most cell types. Adipose-derived stem cell and SVF samples can be counted as live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.

Designed for cost-efficient and accurate cell counting, the LUNA Reusable Slide has the affordability of manual cell counting without the associated subjectivity and time. Photon Slides are disposable precision slides made with optical quality materials for optimal fluorescence signal detection. The slides offer the ultimate counting experience with no mess or clean up, while maintaining the highest standard of cell counting accuracy.

Contact us for a demo today!

TWO COUNTERS IN ONE
LUNA-STEM can count in both brightfield and fluorescence mode. Built-in software automatically generates cell viability data upon cell counting. For validation purposes, live and dead cells are tagged with green and red circles, respectively

COMPATIBLE WITH STEM CELLS & CELLS FROM STROMAL VASCULAR FRACTION (SVF)
The various cells within the SVF can be recognised and counted as non-nucleated cells or live and dead nucleated cells simultaneously, which may not be possible with other cell counters.

FAST COUNTING & IMAGING
Images generated from three channels in 30-60 seconds.

USER-FRIENDLY INTERFACE
Customise and save protocols, verify results immediately onboard, use the dilution calculator, and gate cells on histograms with a few taps on the LUNA-STEM touchscreen.

SIMPLE RECORDKEEPING
Print data with an external printer or export high-resolution images, data and full data reports via USB.

Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue. 2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.

Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation. 2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.

Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials. 2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.

Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia. 2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.

The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging. 2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.

LUNA-FX7 Automated cell counter is our most powerful cell counter with autofocusing, dual fluorescence and brightfield detection plus 21 CFR PART 11 compliance.

Manufacturer	Logos Biosystems
Product Series	LUNA
Measurement principle	Microscopy – Cell image analysis
Application	Live cell image analysis
Cell size range	1 to 90 μm
Optics	Brightfield, Dual Fluorescence
Ex/Em wavelength	470±20 nm/ 530±25 nm, 600 nm

LUNA-FX7 is an automated, high speed cell counter that provides unmatched accuracy in an easy-to-use workflow.

The intuitive user interface provides rapid, accurate and reliable results in three easy steps: LOAD – COUNT – READ.

LUNA-FX7 gives users the flexibility to analyse up to 8 eight samples simultaneously by using the 8-channel slide, with a maximum counting volume of 5 µL in the single-channel slide (10 times that of conventional cell counters). Using brightfield and dual fluorescent detection, precision autofocus, advanced optics and a clever declustering algorithm, the LUNA-FX7 delivers reliable results for almost every cell type. Its broad cell detection range, 1 x 10^4 cells/mL to 5 x 10^7 cells/mL eliminates the need to dilute or concentrate samples, making the system suitable for diverse cell counting applications including single-cell sequencing and CAR-T cell therapy.

To help monitor and optimise bioprocesses, the LUNA-FX7 has unique built-in quality control features with precision validation slides and is 21 CFR Part 11 ready. Cell number concentrations and viabilities are provided with a single tap on the screen. Multiple devices in various locations can be accessed remotely using complementary CountWire software.

The LUNA family of automated counters has been awarded a Diamond seal of quality from SelectScience in recognition of outstanding feedback received from scientists globally. Logos Biosystems are the first to hold this exclusive Seal of Quality and earned this new award for having received over 500 reviews with a very respectable average star rating of 4.8 out of 5.

East-to-use, Accurate, High speed cell counting
Advanced optics and intelligent declustering algorithm
High speed precision autofocus
Multiple slide options (8-, 3-, 2-, 1-chamber formats)
Data transfer via Wi-Fi, USB device, or Ethernet
COUNTWIRE software compliant with 21CRF PART11/GMP requirements
New bioprocess monitoring feature and validation slide for QC
Platinum seal of quality from SelectScience

LUNA-FX7 has a broad cell detection range, 1 x 10^4 cells/mL to 5 x 10^7 cells/mL that eliminates the need to dilute or concentrate samples, making it suitable for diverse cell counting applications including single-cell sequencing and CAR-T cell therapy.

Monitoring CAR-T Cell Therapy Production Using the LUNA-FX7

Rapid evaluation of PBMC counting and viability

The Counting Accuracy of the LUNA-FX7™ Automated Cell Counter

Cell Lines validated
On the LUNA-FX7 Automated cell counter
Cell Type	Animal	Organ
3T3-L1	Mouse	Embryo
A549	Human	Lung
AsPC-1	Human	Pancreas
BAEC	Cow	Aorta
Colon26L5	Mouse	Colon Carcinoma
DLD-1	Human	Colon
H1975	Human	Lung
HCT116	Human	Colon
Hek-293	Human	Embryonic Kidney
HL60	Human	Peripheral Blood
J774A.1	Mouse	Ascites
RAW264.7	Mouse	Ascites
SY5Y	Human	Bone marrow
U2OS	Human	Bone
U937	Human	Lymphocyte
K562	Human	Bone marrow
PBMC	Human	Blood
M1	Mouse	Myeloblast

What is the LUNA-FX7 cell counter used for?

The LUNA-FX7 is an automated fluorescence cell counter used for counting and assessing the viability of various cell types in research and bioprocess applications. It supports brightfield and dual fluorescence (green and red channels) counting with optional 21 CFR Part 11 compliance via CountWire software.

How does automated cell counting work?

The LUNA-FX7 uses image-based analysis with precision autofocus and de-clustering algorithms to identify and count individual cells on disposable or reusable slides.

What is the difference between LUNA-II and LUNA-FX7?

The LUNA-FX7 features dual fluorescence channels (green and red) plus brightfield counting, enabling simultaneous GFP/RFP analysis and multi-parameter cell assays.

Can LUNA-FX7 measure cell viability?

Yes. The LUNA-FX7 supports fluorescence-based viability assessment using its dual green and red fluorescence channels, which are compatible with common live/dead viability dyes for simultaneous cell discrimination in a single measurement.

How accurate is automated cell counting?

The LUNA-FX7 achieves a coefficient of variation (CV) of less than 1%, enabled by a measuring volume of up to 5.1 uL, improved de-clustering algorithms, and precision autofocus.

What cell types can LUNA-FX7 count?

The LUNA-FX7 is designed for high accuracy, high throughput applications including CAR-T cell therapy, bioprocess monitoring, and quality control. It can count PBMCs, splenocytes, yeast, insect cells, protoplasts, sperm cells, and more.

Can LUNA-FX7 measure GFP transfection efficiency?

Yes. The LUNA-FX7 green fluorescence channel is suited for GFP detection, enabling quantitative measurement of transfection efficiency alongside total cell count.

What is the cell size range for LUNA-FX7?

The LUNA-FX7 can detect cells from 1 to 90 um in diameter, with an optimal range of 3 to 60 um.

Does LUNA-FX7 use disposable slides?

Yes. The LUNA-FX7 supports disposable slide formats in 1-, 2-, 3-, and 8-chamber configurations. Reusable slides and the all-in-one SpectraSlide AP-1 are also available.

Can LUNA-FX7 detect cell aggregates?

Yes. The LUNA-FX7 includes improved de-clustering algorithms specifically designed to handle cell aggregates and improve counting accuracy.

What software does LUNA-FX7 include?

The LUNA-FX7 includes software with multiple counting modes (brightfield, fluorescence, QC), customisable protocols, bioprocess monitoring with growth rate tracking, and built-in QC software. Optional CountWire software is available for regulated environments requiring 21 CFR Part 11 compliance.

What types of illumination does the LUNA-FX7 use?

The LUNA-FX7 offers both brightfield and fluorescence cell counting.

What different slides are available for the LUNA-FX7?

The LUNA-FX7 accommodates multiple slide options: reusable slides, 1-, 2-, 3-, and 8-channel disposable slides, and the SpectraSlide AP-1. The 8-channel slides maximise throughput with multi-channel pipette compatibility. The single-channel slide, with a nearly 5 uL counting volume, promotes increased accuracy and precision. With One and Done slide insertion, the LUNA-FX7 counts all chambers without removing and reinserting the slide.

Can I do AO/PI viability measurements with the LUNA-FX7?

Yes. Acridine Orange/Propidium Iodide (AO/PI) Stain causes viable nucleated cells to fluoresce green and nonviable nucleated cells to fluoresce red. AO/PI can be used to assess cell viability with the LUNA-FX7 automated fluorescence cell counter.

Where can I buy counting slides for the LUNA-FX7?

The LUNA-FX7 slides are available from ATA Scientific. Simply send a request to enquiries@atascientific.com.au

What is the SpectraSlide AP-1?

The SpectraSlide AP-1 is a disposable cell counting device designed for use with the LUNA-FX7, integrating cell sampling and staining into one convenient tool. Simply insert the slide into your sample, press the Cell Sampling button, then count your sample with no mixing, no mess, and no pipetting.

Does the LUNA-FX7 comply with 21 CFR Part 11?

Yes. The LUNA-FX7 is equipped with CountWire software, which includes user-accessibility management with encrypted activity logs, delivering data securely and remaining compliant with FDA 21 CFR Part 11 regulations and GMP requirements.

Does the LUNA-FX7 support IQ/OQ validation?

Yes. IQ/OQ documentation is available for the LUNA-FX7.

How does the LUNA-FX7 handle QC?

The LUNA-FX7 includes a QC feature with pre-fixed fluorescent or brightfield validation slides. QC functions allow you to store, monitor, and analyse instrument performance over time. By establishing user-defined acceptance limits, automated tracking and charting functions help ensure compliance with rigorous QA/QC criteria.

Can I have a demonstration or free trial of the LUNA-FX7?

Yes, if a system is available. Contact us for a booking. We can arrange a personalised demo and test your samples within our lab facility in Sydney. Onsite free trials are also available. Contact us or complete a request a demo form to get started.

Automated & affordable cell counter
Brightfield & Dual fluorescence
Manual focus
Cell diameter range: 1 – 90 μm

Fluorescence cell counter for SVF derived- cells
Dual fluorescence
Manual focus
Cell diameter range: 1 – 90 μm

Automated, unmatched accuracy for variety of applications and/or regulatory needs
Brightfield & Dual fluorescence
Auto Focus & Manual Focus
Cell diameter range: 1-90 μm

LUNA FL	LUNA STEM	LUNA FX7
Automated & affordable cell counter	Fluorescence cell counter for SVF derived- cells	Cell counter for wide variety of applications
Brightfield & Dual fluorescence	Dual fluorescence	Brightfield & Dual fluorescence
Manual focus	Manual focus	Auto Focus & Manual Focus
Cell types
Cell lines Primary cells Blood cells PBMCs Splenocyte Thymocyte Yeast cells GFP expressing cells	Stem cells SVF-derived cells (Nucleated and Non-nucleated)	Cell lines Primary cells Whole blood PBMCs Splenocyte Thymocyte Yeast cellsGFP expressing cells
Compatible Dyes
Trypan blue Erythrosin B Acridine orange (AO) Propidium iodide (PI) Fluorescein diacetate	Acridine orange (AO) Propidium iodide (PI)	Trypan blue Erythrosin B Acridine orange (AO) Propidium iodide (PI) Fluorescein diacetate
Liquid lens	Liquid lens	Advanced microscopy lens
Sample loading volume
10 μL	10 μL	10 μL-50 μL (depends on slide type)
Maximum analysis volume
1.3 μL	1.3 μL	0.5 μL-5.1 μL (depends on slide type)
Cell Concentration Range
5×10⁴ – 1×10⁷cells/mL	5×10⁴ – 1×10⁷cells/mL	1 x 10⁴ – 5 x 10⁷ cells/mL
Slide Options
PhotonSlide™ LUNA™ Reusable Slide	PhotonSlide™ LUNA™ Reusable Slide	LUNA™ 1-Channel Slide LUNA™ 3-Channel Slide LUNA™ 8-Channel SlideLUNA™ Cell Counting Slide/ PhotonSlidesLUNA™ Reusable SlideSpectraSlide® (cell sampling & staining in one)
Onboard storage
–	–	250GB/1TB
–	–	21 CFR Part 11 Compliance

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