Sizing nanograms of proteins
The Zetasizer µV provides rapid characterization of very small quantities of proteins. Utilising the highly sensitive Single Mode Fibre (SMF) technology enables measurement of dilute solutions of small proteins, detecting down to a hydrodynamic radius of 0.3 nm. Only 2µl. of protein solution is required.
The Zetasizer µV incorporates both Dynamic and Static light scattering. Dynamic light scattering provides accurate determination of molecular size and weight as well as detecting the presence and proportion of aggregates. Static light scattering enables the determination of absolute molecular weight and 2nd virial coefficient - a value indicating how well the protein is likely to stay in solution.
Click here to contact usHardware features
- Fully automated measurements.
- Minimum sample volume 2µl.
- Sensitivity - only 40 nanograms of BSA is required for sizing.
- Sample fully recoverable
- Temperature control 2 to 90°± 0.1°C.
- Automatic temperature trend for melting point determination.
- Auto-attenuator for optimum measurement conditions.
Intelligent software
The software guides users in developing standard operating procedures (SOPs) for repeatable measurements. It incorporates protein specific reports to assist with the interpretation of data. Any measured parameter can be plotted against any other measured parameter eg time vs. temperature.
A set of utilities includes a molecular weight plotter, estimated molecular weight calculator, shape estimate calculator, concentration calculator and scattering functions plotter.
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