Rapid imaging of particles and cells

FlowCAM is an integrated system that counts, sizes and images particles and cells in discrete samples or in continuous flows. It is a multi function system combining the capabilities of flow cytometry, microscopy, imaging and fluorescence.

FlowCAM integrates multiple measurements into one easy to use system.
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Particles or cells are suspended in a liquid (usually water) which is pumped through a flow cell. The patented optics and CCD camera magnify and capture the image of each particle or cell and its size and shape are measured. Measurements include length, width, area, light scatter and fluorescence.

Function FlowCAM Cytometry Microscopy Fluoroscopy
Particle size and count Yes Yes No No
Imaging Yes No Yes No
In situ sampling Yes No No Yes

An interactive scattergram enables immediate recall of the images and a review of user selected distributions. Three visualisation modes and four levels of magnification allow users to target specific particles or cell types from one micron and above.

FlowCAM uses a powerful new method for visually processing images. Regions of interest in the scattergram are selected with the cursor and all images meeting that criteria are displayed. A "target object " can be chosen by clicking on its image and its values used to define filter parameters. Using the filter with pattern recognition software similar objects can be identified and displayed. A library of species specific filters can be stored for screening future samples.

The speed of imaging and multi measurement capability replaces many hours of tedious microscope work. Rapid imaging of a fluid stream is a powerful means of monitoring processes, especially where specific shapes need to be detected and quantified.

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Applications include

Water Analysis.oceanographic and water applications such as monitoring phytoplankton, ballast water, sediments and algal blooms.

Formulations.characterising solids including cosmetic topicals, pharmaceutical suspensions, flavour carriers, pigments and ink jet dyes

Biotech .discrete or continuous monitoring of enzyme and fermentation processes.

Process monitoring.such as oil in water (produced water), emulsions and dispersions used in the chemical, coatings, polymer and pharmaceutical industries.

Digital imaging of each particle along with size and shape distributions

New High Precision Syringe Pump for FlowCAM®

The FlowCAM® is now available with a high-precision Syringe pump, which can be mounted internally or externally. This enables precise control of sample delivery for applications such as enumeration where absolute control over flow amount and speed are critical. Samples as small as 50μl can be analysed, with sampling efficiencies as high as 90%. Combined with specialised Field of View (FOV) flow cells and automated pump control through the VisualSpreadsheet © software, FlowCAM can achieve maximum volume efficiency with samples as small as 35μl.

The automated process enables the operator to load the sample, press start and walk away while the sample runs unattended. The FlowCAM allows control over flow rate and throughput volume to ensure the entire sample is presented to the flow cell for accurate real-time detection, imaging, counting and measurement of particles and microorganisms.

Benchtop FlowCAM with Syringe Pump installed (left), and external syringe pump option (right)

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