Accurate molecular size and weight
Triple detector GPC
ViscotekViscotek 302-040 Triple Detector
Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography, is a technique that employs columns to separate polymers and proteins according to their hydrodynamic volume in order to determine molecular size and weight.
Click here to contact usIn conventional GPC the separated molecules are measured by a concentration detector (RI or UV/VIS). Molecular weights are determined by calibrating the column using standards of known molecular weight. The drawback is that GPC separation is based on Hydrodynamic Volume, not Molecular Weight. Therefore it is assumed that the sample is of the same density as the standards, which is often not the case. Hence the results are referred to as Relative Molecular Weight. To overcome this calibration limitation, Triple Detection has become the preferred measurement method. It employs a concentration detector, a viscometer and a light scattering detector acting in concert.
- The light scattering detector provides a direct measurement of molecular weight and eliminates the need for column calibration.
- The viscometer detector provides a direct measurement of intrinsic viscosity and allows the determination of molecular size, conformation and structure.
- Concentration is measured with an RI or UV, and is necessary for the determination of both molecular weight and intrinsic viscosity.
Triple detection only requires a single standard for the calibration of all three detectors as well as performing corrections for inter-detector shift and peak broadening effects. Triple detection has a unique advantage in that it provides absolute molecular weight, molecular size (to less than 1 nm) and intrinsic viscosity, as well as information on conformation, branching and aggregation. It is the only technique capable of giving this comprehensive collection of data on-line in a single experiment.
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