A Guide to Modern HPLC

HPLC stands for high performance liquid chromatography. It is a chromatographic technique that can separate a mixture of compounds. This technique is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of the mixture, particularly in the separation of amino acids and proteins due to their different behaviour in solvents related to the amount of electronic charge of each one.

Like liquid chromatography, HPLC uses a liquid mobile phase to transport the sample mixture. However, HPLC is a step up from liquid chromatography in several ways.
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